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Moderator: Matt

By jkochuni
Posts:  625
Joined:  Sun Mar 22, 2009 8:19 pm
#9537
After 2 1/2 weeks my cultures show no signs of contamination, but a few of the cultures have slight blackening on the ends (here are a few of the cultures)...

Dingley Giant and Yellow with no blackening...
Image
Image

Blackening ends...
Image

Sawtooth that has me worried...
Image

I think that they will make it since they have only blackened slightly in two weeks. Has anyone tried cutting the ends off? I'm worried it could infect them though.

Sorry for the crappy photos.
Last edited by jkochuni on Wed May 06, 2009 12:06 am, edited 2 times in total.
By kevinqwe
Posts:  1840
Joined:  Tue Mar 31, 2009 3:04 am
#9540
what kind of is the water in there?
why dont you just plant the stem instead of putting it in water?

stem number looks the best ;)
By jkochuni
Posts:  625
Joined:  Sun Mar 22, 2009 8:19 pm
#9543
kevinqwe wrote:what kind of is the water in there?
why dont you just plant the stem instead of putting it in water?

stem number looks the best ;)

Actually it is not water but agar and MS medium. It is used to induce cells in the plant tissue to multiply giving you the possibility of generating numerous plants from one piece of tissue.
By hackerberry
Location: 
Posts:  1704
Joined:  Fri Feb 13, 2009 7:58 pm
#9556
Did you add BAP in your medium? It will help your tissue develop shoots before it totally die off.

hb
By kevinqwe
Posts:  1840
Joined:  Tue Mar 31, 2009 3:04 am
#9569
jkochuni ,
where do you get it from?
By jkochuni
Posts:  625
Joined:  Sun Mar 22, 2009 8:19 pm
#9655
Matt wrote:Looking good Jason! I can't wait to get home and check on my cultures. I haven't really seen them in 3 weeks now!
I bet they are growing like mad!
By jkochuni
Posts:  625
Joined:  Sun Mar 22, 2009 8:19 pm
#9657
hackerberry wrote:Did you add BAP in your medium? It will help your tissue develop shoots before it totally die off.

hb

I did not add BAP, but I just ordered some today from Carol. I will try and replate a few of the cultures. I hope several will produce shoots regardless.
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