White film plaque on tissue culture medium but not growing

White film plaque on tissue culture medium but not growing#320317

By SSCelik -  Sat Aug 18, 2018 3:11 pm
Posts:  1
Joined:  Sat Aug 18, 2018 3:06 pm

- Sat Aug 18, 2018 3:11 pm #320317

I am a hobbyist at plant tissue culture, I preparing my MS medium by formula (from chemicals) and I am getting a white film layer on the surface of medium after 7-8 days of incubation. I am not sure that it is contamination becuase it is not growing.

Some times getting a brown/yellow film on top of medium. it is not growing also.

Should I suspect my methods or my chemicals.. All my chemicals is %99 chem pure.

Thanks for your help.

Re: White film plaque on tissue culture medium but not growi#320533

By TissueBob -  Wed Aug 22, 2018 6:39 am
Posts:  8
Joined:  Sun Jan 07, 2018 7:43 pm

- Wed Aug 22, 2018 6:39 am #320533

it is contamination. media was not good sterilized.

Re: White film plaque on tissue culture medium but not growi#321849

By MattByers -  Mon Sep 10, 2018 4:52 am
Posts:  16
Joined:  Tue Aug 28, 2018 2:46 am

- Mon Sep 10, 2018 4:52 am #321849

I agree with Bob, you've got to bring it to 18 psi (255F) for 15 minutes.

Matt

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