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Discuss all micropropagation related topics here.

Moderator: Matt

By gage
Posts:  170
Joined:  Thu Sep 17, 2009 8:23 pm
#28817
yea haha it just seemed like there was something a little more to it
By gage
Posts:  170
Joined:  Thu Sep 17, 2009 8:23 pm
#28818
also what other types of media would i use for a tc of a different plant
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By Matt
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Posts:  22531
Joined:  Mon Apr 21, 2008 11:28 pm
#28823
gage wrote:also what other types of media would i use for a tc of a different plant
It completely depends on the plant. To my knowledge almost any plant can be grown on one of three types of media:
* Murashige and Skoog
* Knudson C
* Woody Plant
Depending on what kind of plant you want to TC, you'll have to research what kind of media is best.
By gage
Posts:  170
Joined:  Thu Sep 17, 2009 8:23 pm
#28898
okay thanks i will have to do so once i get my tc going
By gage
Posts:  170
Joined:  Thu Sep 17, 2009 8:23 pm
#28955
do you need a pressure cooker with a gauge, where would be a cheap place to buy one with a gauge?
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By Matt
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Posts:  22531
Joined:  Mon Apr 21, 2008 11:28 pm
#28982
gage wrote:do you need a pressure cooker with a gauge, where would be a cheap place to buy one with a gauge?
I wondered the same thing when I started. No, you don't need one with a gauge. Almost all pressure cookers are set to reach and maintain a pressure of 15 PSI, which is what you want when sterilizing media.
By gage
Posts:  170
Joined:  Thu Sep 17, 2009 8:23 pm
#28989
alright thanks, that will save me around 50 bucks
By Aging_Bourbon
Location: 
Posts:  2799
Joined:  Wed Dec 31, 2008 10:14 pm
#29140
Hello ya'll,

well I'm still interested in doing TC and I read the FAQ Sticky but is their other thing's that I should know?



Allen,
By hackerberry
Location: 
Posts:  1704
Joined:  Fri Feb 13, 2009 7:58 pm
#29143
Be Patient hehe. Seriously, there will be tons of questions when you actually start on your TC and not all get the results that everyone else gets. You will develop your own technique as you progress. Like me, I skipped PH testing and scaling. I already created my own method to make things faster to make. I just started using plain Jello instead of agar. Much cheaper but gives you the same result. That's just me.

hb
By gage
Posts:  170
Joined:  Thu Sep 17, 2009 8:23 pm
#29165
hackerberry wrote:Be Patient hehe. Seriously, there will be tons of questions when you actually start on your TC and not all get the results that everyone else gets. You will develop your own technique as you progress. Like me, I skipped PH testing and scaling. I already created my own method to make things faster to make. I just started using plain Jello instead of agar. Much cheaper but gives you the same result. That's just me.

hb

So instead of agar you use gelatin, pectin or jello?
By hackerberry
Location: 
Posts:  1704
Joined:  Fri Feb 13, 2009 7:58 pm
#29171
Yes gage, they do me the same purpose but on the cheaper side.

hb
By Aging_Bourbon
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Posts:  2799
Joined:  Wed Dec 31, 2008 10:14 pm
#29172
Jello as in Instant jello?
By hackerberry
Location: 
Posts:  1704
Joined:  Fri Feb 13, 2009 7:58 pm
#29174
Jello man....That's what I used in the first place when I was just starting. The sole purpose of Agar is for cell suspension which Jellos do.

hb
By Aging_Bourbon
Location: 
Posts:  2799
Joined:  Wed Dec 31, 2008 10:14 pm
#29176
oh i see now lol.
By gage
Posts:  170
Joined:  Thu Sep 17, 2009 8:23 pm
#29207
alright, do you know if they raise or lower the ph very much?
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